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antibody stripping buffer  (TaKaRa)


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    Structured Review

    TaKaRa antibody stripping buffer
    Antibody Stripping Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibody+stripping+buffer/Western+BLoT+Stripping+Buffer/pm41634752-122-12-18
    Average 95 stars, based on 77 article reviews
    antibody stripping buffer - by Bioz Stars, 2026-10
    95/100 stars

    Images

    Related Articles

    Membrane:

    Article Title: Identification of serine/arginine-rich splicing factor 1-interacting proteins in Plasmodium berghei
    Article Snippet: Chemiluminescence was generated using Chemi-Lumi One Super (Cat. No. 02230-14, NACALAI TESQUE, Inc., Kyoto, Japan), and the signal was captured using the ChemiDoc Touch Imaging System (Cat. No. 1708370J1PC, Bio-Rad Laboratories, Inc., Tokyo, Japan). .. Following detection of the mCherry-fused Rhop2 protein, the membrane was incubated in antibody stripping buffer (Cat. No. T7135A, TaKaRa Bio Inc., Shiga, Japan) for 3 h at room temperature with gentle shaking. .. After several washes with TBS-T, the membrane was subjected to detection of GFP-fused SR1 using anti-GFP antibody (1:1000 dilution, Cat. No. 668205, BioLegend, CA, USA) as the primary antibody for 3 h at room temperature and rabbit anti-mouse IgG-HRP (1:5000 dilution, Cat. No. AP160P, Merck Millipore, MA, USA) as the secondary antibody for 1 h at room temperature.

    Article Title: Identification of serine/arginine-rich splicing factor 1-interacting proteins in Plasmodium berghei.
    Article Snippet: 21 4 5 41 48 .5 5 28 .5 2 20 12 5 1708370J1PC, Bio-Rad Laboratories, Inc., Tokyo, Japan). .. Following detection of the mCherry-fused Rhop2 protein, the membrane was incubated in antibody stripping buffer (Cat. No. T7135A, TaKaRa Bio Inc., Shiga, Japan) for 3 h at room temperature with gentle shaking. .. After several washes with TBS-T, the membrane was subjected to detection of GFP-fused SR1 using anti-GFP antibody (1:1000 dilution, Cat. No. 668205, BioLegend, CA, USA) as the primary antibody for 3 h at room temperature and rabbit anti-mouse IgG-HRP (1:5000 dilution, Cat. No. AP160P, Merck Millipore, MA, USA) as the secondary antibody for 1 h at room temperature.

    Incubation:

    Article Title: Identification of serine/arginine-rich splicing factor 1-interacting proteins in Plasmodium berghei
    Article Snippet: Chemiluminescence was generated using Chemi-Lumi One Super (Cat. No. 02230-14, NACALAI TESQUE, Inc., Kyoto, Japan), and the signal was captured using the ChemiDoc Touch Imaging System (Cat. No. 1708370J1PC, Bio-Rad Laboratories, Inc., Tokyo, Japan). .. Following detection of the mCherry-fused Rhop2 protein, the membrane was incubated in antibody stripping buffer (Cat. No. T7135A, TaKaRa Bio Inc., Shiga, Japan) for 3 h at room temperature with gentle shaking. .. After several washes with TBS-T, the membrane was subjected to detection of GFP-fused SR1 using anti-GFP antibody (1:1000 dilution, Cat. No. 668205, BioLegend, CA, USA) as the primary antibody for 3 h at room temperature and rabbit anti-mouse IgG-HRP (1:5000 dilution, Cat. No. AP160P, Merck Millipore, MA, USA) as the secondary antibody for 1 h at room temperature.

    Article Title: Identification of serine/arginine-rich splicing factor 1-interacting proteins in Plasmodium berghei.
    Article Snippet: 21 4 5 41 48 .5 5 28 .5 2 20 12 5 1708370J1PC, Bio-Rad Laboratories, Inc., Tokyo, Japan). .. Following detection of the mCherry-fused Rhop2 protein, the membrane was incubated in antibody stripping buffer (Cat. No. T7135A, TaKaRa Bio Inc., Shiga, Japan) for 3 h at room temperature with gentle shaking. .. After several washes with TBS-T, the membrane was subjected to detection of GFP-fused SR1 using anti-GFP antibody (1:1000 dilution, Cat. No. 668205, BioLegend, CA, USA) as the primary antibody for 3 h at room temperature and rabbit anti-mouse IgG-HRP (1:5000 dilution, Cat. No. AP160P, Merck Millipore, MA, USA) as the secondary antibody for 1 h at room temperature.

    Stripping Membranes:

    Article Title: Identification of serine/arginine-rich splicing factor 1-interacting proteins in Plasmodium berghei
    Article Snippet: Chemiluminescence was generated using Chemi-Lumi One Super (Cat. No. 02230-14, NACALAI TESQUE, Inc., Kyoto, Japan), and the signal was captured using the ChemiDoc Touch Imaging System (Cat. No. 1708370J1PC, Bio-Rad Laboratories, Inc., Tokyo, Japan). .. Following detection of the mCherry-fused Rhop2 protein, the membrane was incubated in antibody stripping buffer (Cat. No. T7135A, TaKaRa Bio Inc., Shiga, Japan) for 3 h at room temperature with gentle shaking. .. After several washes with TBS-T, the membrane was subjected to detection of GFP-fused SR1 using anti-GFP antibody (1:1000 dilution, Cat. No. 668205, BioLegend, CA, USA) as the primary antibody for 3 h at room temperature and rabbit anti-mouse IgG-HRP (1:5000 dilution, Cat. No. AP160P, Merck Millipore, MA, USA) as the secondary antibody for 1 h at room temperature.

    Article Title: Identification of serine/arginine-rich splicing factor 1-interacting proteins in Plasmodium berghei.
    Article Snippet: 21 4 5 41 48 .5 5 28 .5 2 20 12 5 1708370J1PC, Bio-Rad Laboratories, Inc., Tokyo, Japan). .. Following detection of the mCherry-fused Rhop2 protein, the membrane was incubated in antibody stripping buffer (Cat. No. T7135A, TaKaRa Bio Inc., Shiga, Japan) for 3 h at room temperature with gentle shaking. .. After several washes with TBS-T, the membrane was subjected to detection of GFP-fused SR1 using anti-GFP antibody (1:1000 dilution, Cat. No. 668205, BioLegend, CA, USA) as the primary antibody for 3 h at room temperature and rabbit anti-mouse IgG-HRP (1:5000 dilution, Cat. No. AP160P, Merck Millipore, MA, USA) as the secondary antibody for 1 h at room temperature.

    Gentle:

    Article Title: Identification of serine/arginine-rich splicing factor 1-interacting proteins in Plasmodium berghei
    Article Snippet: Chemiluminescence was generated using Chemi-Lumi One Super (Cat. No. 02230-14, NACALAI TESQUE, Inc., Kyoto, Japan), and the signal was captured using the ChemiDoc Touch Imaging System (Cat. No. 1708370J1PC, Bio-Rad Laboratories, Inc., Tokyo, Japan). .. Following detection of the mCherry-fused Rhop2 protein, the membrane was incubated in antibody stripping buffer (Cat. No. T7135A, TaKaRa Bio Inc., Shiga, Japan) for 3 h at room temperature with gentle shaking. .. After several washes with TBS-T, the membrane was subjected to detection of GFP-fused SR1 using anti-GFP antibody (1:1000 dilution, Cat. No. 668205, BioLegend, CA, USA) as the primary antibody for 3 h at room temperature and rabbit anti-mouse IgG-HRP (1:5000 dilution, Cat. No. AP160P, Merck Millipore, MA, USA) as the secondary antibody for 1 h at room temperature.

    Article Title: Identification of serine/arginine-rich splicing factor 1-interacting proteins in Plasmodium berghei.
    Article Snippet: 21 4 5 41 48 .5 5 28 .5 2 20 12 5 1708370J1PC, Bio-Rad Laboratories, Inc., Tokyo, Japan). .. Following detection of the mCherry-fused Rhop2 protein, the membrane was incubated in antibody stripping buffer (Cat. No. T7135A, TaKaRa Bio Inc., Shiga, Japan) for 3 h at room temperature with gentle shaking. .. After several washes with TBS-T, the membrane was subjected to detection of GFP-fused SR1 using anti-GFP antibody (1:1000 dilution, Cat. No. 668205, BioLegend, CA, USA) as the primary antibody for 3 h at room temperature and rabbit anti-mouse IgG-HRP (1:5000 dilution, Cat. No. AP160P, Merck Millipore, MA, USA) as the secondary antibody for 1 h at room temperature.



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